Cloned (Comment) | Organism |
---|---|
gene VF_A0360, genotyping, overview, cloning of plasmid pTn7BinK | Aliivibrio fischeri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme-deficient mutant strain rscS*DELTAbinK sypG::Tnerm, competition assays of wild-type and mutant strains in vitro and in vivo, overview. DELTAbinK has no effect in the pSypG sypE+ background | Aliivibrio fischeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | Q5E0L6 | - |
- |
Aliivibrio fischeri ATCC 700601 | Q5E0L6 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
BinK | - |
Aliivibrio fischeri |
sensor histidine kinase | - |
Aliivibrio fischeri |
VF_A0360 | - |
Aliivibrio fischeri |
General Information | Comment | Organism |
---|---|---|
malfunction | binK mutants exhibit a colonization advantage in a global genetic screen, phenotype, overview. Bacterial biofilm aggregates in the host are larger in strains lacking BinK, whereas overexpression of BinK suppresses biofilm formation and squid colonization | Aliivibrio fischeri |
physiological function | gene VF_A0360 encodes the biofilm inhibitor kinase binK, an orphan hybrid histidine kinase that negatively regulates the Vibrio fischeri symbiotic biofilm (Syp) in vivo and in vitro. Colonization of Euprymna scolopes bobtail squid by Vibrio fischeri bacteria requires bacterial aggregation in host mucus as the symbiont transitions from a planktonic lifestyle in seawater to a biofilm-associated state in the host. Enzyme BinK acts upstream of SypG, the sigma54-dependent transcriptional regulator of the syp biofilm locus. The BinK effects are dependent on intact signaling in the RscS-Syp biofilm pathway. Enzyme BinK antagonizes the signal from RscS and serves as an integral component in Vibrio fischeri biofilm regulation. The hybrid histidine kinase BinK is a negative regulator of in vivo aggregation and squid colonization and of Syp biofilm phenotypes in culture. BinK is acting through SypG and not SypE | Aliivibrio fischeri |